Identification of yeast by MALDI-TOF-MS
(Matrix-assisted laser desorption/ionization Time of Flight Mass spectrometry)
Identification of yeast by MALDI-TOF-MS(Matrix-assisted laser desorption/ionization Time of Flight Mass spectrometry)Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is a tool that has recently been established for the microbial identification of fungi and bacteria, mainly in clinical environments.
A laser is used to ionize proteins in cell samples that are embedded in a crystalline matrix, and after acceleration in an electric field the proteins can be detected, generating protein mass spectra. These mass spectra can be compared to database entries to identify microorganisms usually up to the species level. The analysis by this method is faster than other techniques that rely on metabolical or biochemical tests.
In cooperation with the VLB Berlin and the company NovaBiotec, the aim of this project is to establish a process that is tailored to the specific needs of the yeast
The goal is to allow the identification/differentiation of wild, spoilage and culture yeasts, providing a simple sampling and stabilization method that will generate
reproducible results. In a second step, it will be evaluated to what extent process metabolical states of yeast can be associated with measured spectra, offering additional process monitoring data.